This course is organized as an advanced-level explanation of molecular biology techniques for professionals in genetics and bioengineering. Apart from basic techniques, such as DNA, RNA, and protein isolation, spectrophotometry, electrophoresis, and PCR, advanced topics in molecular biology are introduced as well. Some of those topics are: primer design, two-dimensional electrophoresis, blotting techniques, in situ hybridization, etc. Occasional practical sessions are organized in order to remind graduates on the basic postulates of laboratory work in molecular biology.

Course Objectives

·         Illustrating different methods of isolation of nucleic acids and proteins.

·         Revising the advantages and drawbacks of different quantification methods.

·         Explaining graduates how to perform analysis of different biomolecules practically.

·         Introduction to primer design.

·         Explaining how to prepare radioactively-labeled nucleic acid probes and how to perform blotting techniques.

·         Introduction to NGS.

  Course Content(weekly plan)

Week 1: Introduction to the course

Week 2: DNA and RNA isolation methods

Week 3: Protein isolation methods

Week 4: UV/vis spectrophotometry

Week 5: Vertical and horizontal electrophoresis

Week 6: Two-dimensional electrophoresis

Week 7: Thermal cycling (PCR and qPCR)


Week 9: Primer design

Week 10: Preparation of radiolabeled DNA and RNA probes

Week 11: Southern, Northern, and Western blotting

Week 12: In situ hybridization

Week 13: Restriction digestion and ligation of DNA fragments

Week 14: Bacterial transformation: Plasmid isolation, vectors, and plasmid insertion

Week 15: Next-generation sequencing