This course is organized as an advanced-level explanation of molecular biology techniques for professionals in genetics and bioengineering. Apart from basic techniques, such as DNA, RNA, and protein isolation, spectrophotometry, electrophoresis, and PCR, advanced topics in molecular biology are introduced as well. Some of those topics are: primer design, two-dimensional electrophoresis, blotting techniques, in situ hybridization, etc. Occasional practical sessions are organized in order to remind graduates on the basic postulates of laboratory work in molecular biology.
Course Objectives
·
Illustrating different methods of isolation of
nucleic acids and proteins. ·
Revising the advantages and drawbacks of different
quantification methods. ·
Explaining graduates how to perform analysis of different
biomolecules practically. ·
Introduction to primer design. ·
Explaining how to prepare radioactively-labeled
nucleic acid probes and how to perform blotting techniques. · Introduction to NGS.
Week 1:
Introduction to the course Week 2:
DNA and RNA isolation methods Week 3:
Protein isolation methods Week 4:
UV/vis spectrophotometry Week 5:
Vertical and horizontal electrophoresis Week 6:
Two-dimensional electrophoresis Week 7:
Thermal cycling (PCR and qPCR) WEEK 8: MID-TERM EXAM Week 9:
Primer design Week 10:
Preparation of radiolabeled DNA and RNA probes Week 11:
Southern, Northern, and Western blotting Week 12: In situ hybridization Week 13:
Restriction digestion and ligation of DNA fragments Week 14:
Bacterial transformation: Plasmid isolation, vectors, and plasmid insertion Week 15: Next-generation
sequencing Week 16: FINAL EXAM
- Teacher: Larisa Besic
- Teacher: Amina Kurtovic